Hipure Rna Column Viral Rna Column

• Transport Package: Box
• Specification: 2ml
• Trademark: Magen Biotech
• Origin: China
• Model NO.: R50011
• HS Code: 3926909090

Product Description

Product Description

Introduction

The HiPure DNA/RNA Isolation Columns are designed for isolation of DNA/RNA for biological samples. DNA/RNA is bound to the silica membrane spin columns in the presence of high concentrations of chaotrophic salts, contaminants are washed away, and RNA/DNA is eluted from the silica membrane in water or low-salt buffer. Our membranes are made of binder-free microbifer glass with defined pore size and thickness, both of which are critical for efficient nucleic acid binding. We have validated these membranes for effective binding of both DNA and RNA, in the presence of chaotrophic and non-chaotrophic salts. Cat. No. & Specifications.




Binding Mechanism
The principle of silica matrices purification is based on high affinity of negatively charged DNA backbone towards the positively charged silica particles.  DNA/RNA binds to silica through hydrogen-binding interacation with an underivatised hydrophilic matrix provided by silica, under concentrated chaotrophic salt conditions.  Sodium plays a role as a cation bridge that attracts the negatively charged oxygen ions in silica under high salt conditions (pH ≤ 7.00).  DNA/RNA is tightly bound, and extensive washing removes all contaminants. 

Under high salt concentrations, nucleic acids selectively bind to silica membranes while other contaminants, mainly proteins, pass through the membranes. By and large, guanidinium thiocyanate and guanidinium hydrochloride are used for binding nucleic acids to silica membranes.  Guanidinium thiocyanate at a concentration of 4M ot 6M works best, while guanidinium hydrochloride is used at higher concentrations (in excess of 6M).  In order to control pH of the binding reagent, sodium acetate and Tris-HCl buffers, ranging from pH 6.00 to 7.50 are used.  Binding of smaller fragments and preferable binding of RNA over DNA appear to be a function of pH and membrane characteristics.  The binding efficiency is significantly improved in the presence of ethanol. 




Applications

1.Plasmid isolation

2.Agarose Gel DNA Extraction and PCR Product Purification

3.Nucleic acid Isolation, Purification and Concentration

Special quality inspection
0.05% column was used for plasmid DNA extraction, and then the binding efficiency and the highest binding force were checked.



Storage and SHiPure RNA Column Viral RNA Column
1. Non-DNA remaining
2. High yield and purity guaranteed, up to 70ug RNA.
3. Very High Performance and Stability for Long-term Storage.
4. High-loading capacity
    no more spill-over and cross contamination
    no extra steps for processing
5. High qualify for RNA purification and has been used extensively in house application and by many academic labs.
6. Compatible with other company buffer (Qiagen, Sigma, Promega ect).
7. Economical for large sample process.shapes.



Specification
Technology:Silica-membrane technology
Format:Mini Spin Column
Fragment Size:> 200 nt
Banding capability:150-200ug
 

Features
1. Plastic centrifuge tube made from high quality PP material 
2. Flat top design together with a large white writing area for sample identification
3. Max rotate speed up to 12,000g

More information for you.
Our spin column is 2ml capacity with silica membrane+white O-Ring , the color is transparent, material is PP.
One spin column with a one collection tube.
We also have sale them separately ,you can choose the product you need.
We are used to pack 1000pcs with a plastic bag and 10bags in one carton box.
 

Packaging & Shipping

 

Certificate

 

Laboratory

Workshop

Fininshed product area