Enzyme Digestion Sequencing HiPure Bacterial DNA Kit Isolation from cultures food

• Customization: Available
• CAS No.: /
• Formula: /
• Model NO.: D314602 / D314603
• EINECS: /
• Classification: Biochemical Reagents
• Grade: Reagent Grade
• Specific Usage: For Biological Purpose, For Microbiological, Isolation bacterial DNA from cultures, food
• Content: Standard
• Usage: Laboratory Reagents, Analytical Reagents, Diagnostic Reagents, Teaching Reagents, PCR, southern blot and enzyme digestion
• Source: Lab
• Habit Appellation: HiPure Bacterial DNA Kit
• Application: Industry, Scientific Research, Health, Environmental Protection, Agriculture
• Property: Biochemical Reagent
• Main Functions: Isolation bacterial DNA from cultures, food
• Applications: PCR, southern blot and enzyme digestion, etc.
• Purification Method: Mini Spin Column
• Purification Technology: Silica Technology
• Process Method: Manual (Centrifugation or Vacuum)
• Sample Type: Culture medium, swab, parasitic blood, tissue
• Sample Amount1: Bacterial culture medium: 0.5-2 ml
• Sample amount2: Tissue samples: 50-100 mg
• Sample amount3: Whole blood / cell suspension: 0.5-1 ml
• Sample amount4: Viscous secretion: 0.1-1g
• Elution Volume: ≥30μl
• Time per run: ≤40 minutes
• Liquid Carrying Volume Per Column: 800μl
• Binding Yield of Column: 100μg
• Transport Package: Bottle/ Box
• Specification: 50 preps / 250 preps
• Trademark: Magen Biotech
• Origin: China
• HS Code: 3822190090
• Production Capacity: 100000 Per Month

Product Description

Product Description

HiPure Bacterial DNA Kit
 

CAT NO	PRODUCT NAME	SIZE	PRICE
D314602	HiPure Bacterial DNA Kit	50 preps	$149.00
D314603	HiPure Bacterial DNA Kit	250 preps	$646.00

This product provides fast and easy methods for purification of total DNA for reliable PCR and southern blotting. Total DNA can be purified from bacterial culture, body fluids, food and fermentation.


Specifications

Features	Specifications
Main Functions	Isolation bacterial DNA from cultures, food and other samples
Applications	PCR, southern blot and enzyme digestion, etc.
Purification method	Mini spin column
Purification technology	Silica technology
Process method	Manual (centrifugation or vacuum)
Sample type	Culture medium, swab, parasitic blood, tissue,sputum, etc.
Sample amount	Bacterial culture medium: 0.5-2 ml Tissue samples: 50-100 mg Whole blood / cell suspension: 0.5-1 ml Viscous secretion: 0.1-1 g
Elution volume	≥30μl
Time per run	≤40 minutes
Liquid carrying volume per column	800μl
Binding yield of column	100μg

Principle
This product is based on silica Column purification. The sample is lysed and digested with lysate and protease, DNA is released into the lysate. Transfer to an adsorption column. Nucleic acid is adsorbed on the membrane, while proteinis not adsorbed and is removed with filtration. After washing proteins and other impurities, Nucleic acid was finally eluted with low-salt buffer (10mmTris, pH9.0, 0.5mm EDTA). This product carries 0.1-0.2mm acid glass beads, which can improve the wall-breaking effect of lysozyme resistant bacteria, the success rate and DNA yield through physical bead grinding.

Advantages
Fast - several samples can be extracted in 40 minutes (after digestion)
High purity - purified DNA can be directly used in various downstream applications
Good repeatability - silica technology can obtain ideal results every time
High recovery - DNA can be recovered at the level of PG
Broad spectrum - it can deal with a variety of bacteria, including Gram-positive bacteria that are difficult to disrupt
Sufficient components - this kit has carried lysozyme, protease K, RNase A and glass beads

Kit Contents

Contents	D314602	D314603
Purification Times	50 Preps	250 Preps
Hipure DNA Mini Columns I	50	2 x 125
2ml Collection Tubes	50	2 x 125
Glass Beads (0.1~0.2mm)	20 g	100 g
Buffer P1	20 ml	100 ml
Buffer DL	15 ml	80 ml
Buffer GW1	22 ml	88 ml
Buffer GW2	12 ml	50 ml
Lysozyme	60 mg	300 mg
Proteinase K	24 mg	120 mg
Protease Dissolve Buffer	5 ml	15 ml
Buffer AE	15 ml	60 ml

Storage and Stability
Lysozyme and Proteinase K should be stored at 2-8°C upon arrival. However, short-term storage (up to 12 weeks) at room temperature (15-25°C) does not affect their performance. The remaining kit components can be stored dry at room temperature (15-25°C) and are stable for at least 18 months under these conditions.

 

Detailed Photos

 

Protocol

 

 

Test Report

 

 

Company Profile

 

 

FAQ

 

Q1.What is the Trade Term?
A1:Ex-work factory (Guangzhou)

Q2. How long is the guarantee (period)?
A2:The product can be stored properly for at least 18 months .

Q3.Are you a manufacturer ? 
A3:Yes, we were established in 2012 and our headquarter is in Guangzhou. All products can be provided with OEM packaging, greatly reduce the cost, and can be flexible in technology and other aspects of communication and cooperation.

Q4.How long is our Production leading time?
A4:Generally our lead time is 2-7 working days after receiving 100% prepayment. If order in bulk , lead time will be longer. Customized bulk quantities are determined by the specific quantity.

Q5.What is the Payment term?
A5:T/T, Paypal, Alipay, Western Union. 100% payment in advance.

Q6.What is the packing details?
A6:The outer packaging of general products is cardboard boxes. Low-temperature products will have ice packs places in foam boxes and then packed into cartons.

Q7. What support you will have to assemble this extractor?
A7: Our extraction instrument is integrated, easy to operate and does not require assembly.

Q8.What kind of documents we will provide to you?
A8:B/L or AWB number, Commercial Invoice, Packing List, MSDS, COA.

Q9. How to apply the free samples?
A9: For free samples we have to know your company name, your E-mail, your FedEx account, and the detail address of your company include the phone number. You can contact with us to apply the free samples.

Q10. Do you accept the small orders?
A10: Yes, we accept the small orders. We will try our best to support our customer's business whether the small trail order.