Cdna Synthesis Snp Site Analysis C Vacuum tumor-specific extracellular DNA Midi Kit

• Customization: Available
• CAS No.: /
• Formula: /
• Model NO.: D318201C / D318202C
• EINECS: /
• Classification: Biochemical Reagents
• Grade: Reagent Grade
• Specific Usage: For Biological Purpose, For Microbiological, Isolation circulating DNA from 1-5ml plasma
• Content: Standard
• Usage: Laboratory Reagents, Analytical Reagents, Diagnostic Reagents, Teaching Reagents, qPCR, liquid or solid chip analysis, hybridization
• Source: Lab
• Habit Appellation: HiPure Circulating DNA Midi Kit C
• Application: Industry, Scientific Research, Health, Environmental Protection, Agriculture
• Property: Biochemical Reagent
• Main Functions: Isolation circulating DNA from 1-5ml plasma
• Main Functions2: Isolation circulating DNA from serum, body fluids
• Applications: qPCR, liquid or solid chip analysis, hybridization
• Purification Method: Mini Spin Column
• Purification Technology: Silica Technology
• Process Method: Manual (vacuum)
• Sample Type: Serum, plasma and other cell-free fluid samples
• Sample Amount: 1-5ml
• Elution Volume: ≥50μl
• Time per run: ≤60 minutes
• Liquid Carrying Volume Per Column: 4ml
• Binding Yield of Column: 1mg
• Transport Package: Bottle/ Box
• Specification: 10 preps / 50 preps
• Trademark: Magen Biotech
• Origin: China
• HS Code: 3822190090
• Production Capacity: 100000 Per Month

Product Description

Product Description

HiPure Circulating DNA Midi Kit C (Vaccum Protocol)
 

CAT NO	PRODUCT NAME	SIZE	PRICE
D318201C	HiPure Circulating DNA Midi Kit C	10 preps	$136.00
D318202C	HiPure Circulating DNA Midi Kit C	50 preps	$547.00

Free-circulating nucleic acids, such as tumor-specific extracellular DNA fragments and mRNAs in the blood or fetal nucleic acids in maternal blood, are present in serum or plasma usually as short fragments, <1000bp (DNA). HiPure Circulating DNA Midi Kit enables efficient purification of these circulating nucleic acids from human plasma, serum, or urine.
This product is for research use only.

Specifications

Features	Specifications
Main Functions	Isolation circulating DNA from 1-5ml plasma, serum, body fluids using vacuum protocol
Applications	qPCR, liquid or solid chip analysis, hybridization and SNP detection, etc.
Purification method	Mini spin column
Purification technology	Silica technology
Process method	Manual (vacuum)
Sample type	Serum, plasma and other cell-free fluid samples
Sample amount	1-5ml
Elution volume	≥50μl
Time per run	≤60 minutes
Liquid carrying volume per column	4ml
Binding yield of column	1mg

Principle
This product is based on silica Column purification. The sample is lysed and digested with lysate and protease, DNA is released into the lysate. Transfer to an adsorption column. Nucleic acid is adsorbed on the membrane, while protein is not adsorbed and is removed with filtration. After washing proteins and other impurities, Nucleic acid was finally eluted with low-salt buffer.

Advantages
High yield - most optimal process, free DNA (>50bp) can be obtained to the maximum extent
High concentration - low elution volume, ensuring high nucleic acid concentration
High purity - low alcohol binding method, completely removing inhibitor and protein pollution
High recovery - DNA can be recovered at thelevel of PG by silica gel column purification

Kit Contents

Contents	D318201C	D318202C
Purification Times	10	50
Buffer ACL	50 ml	250 ml
Buffer ACB*	60 ml	300 ml
Buffer DCW1*	4.4 ml	22 ml
Buffer DCW2*	5 ml	10 ml
Proteinase K	110 ml	540 mg
Protease Dissolve Buffer	10 ml	30 ml
Carrier RNA	110 μg	110 μg
Nuclease Free Water	10 ml	20 ml
HiPure CFDNA Mini Columns	10	50
2 ml Collection Tubes	20	100
Extender Tube	10	50
Vac-Connector	10	50

Storage and Stability
Proteinase K, Carrier RNA should be stored at 2-8°C upon arrival. However, short-term storage (up to 12 weeks) at room temperature (15-25°C) does not affect their performance. The remaining kit components can be stored dry at room temperature (15-25°C) and are stable for at least 18 months under these conditions. The entire kit can be stored at 2-8°C, but in this case buffers should be redissolved before use. Make sure that all buffers are at room temperature when used.

 

Experiment Data



 

Detailed Photos

 

Protocol

 

Test Report

 

 

Company Profile

 

 

FAQ

 

Q1.What is the Trade Term?
A1:Ex-work factory (Guangzhou)

Q2. How long is the guarantee (period)?
A2:The product can be stored properly for at least 18 months .

Q3.Are you a manufacturer ? 
A3:Yes, we were established in 2012 and our headquarter is in Guangzhou. All products can be provided with OEM packaging, greatly reduce the cost, and can be flexible in technology and other aspects of communication and cooperation.

Q4.How long is our Production leading time?
A4:Generally our lead time is 2-7 working days after receiving 100% prepayment. If order in bulk , lead time will be longer. Customized bulk quantities are determined by the specific quantity.

Q5.What is the Payment term?
A5:T/T, Paypal, Alipay, Western Union. 100% payment in advance.

Q6.What is the packing details?
A6:The outer packaging of general products is cardboard boxes. Low-temperature products will have ice packs places in foam boxes and then packed into cartons.

Q7. What support you will have to assemble this extractor?
A7: Our extraction instrument is integrated, easy to operate and does not require assembly.

Q8.What kind of documents we will provide to you?
A8:B/L or AWB number, Commercial Invoice, Packing List, MSDS, COA.

Q9. How to apply the free samples?
A9: For free samples we have to know your company name, your E-mail, your FedEx account, and the detail address of your company include the phone number. You can contact with us to apply the free samples.

Q10. Do you accept the small orders?
A10: Yes, we accept the small orders. We will try our best to support our customer's business whether the small trail order.