Magpure Total Rna Kit

• Customization: Available
• Material: Plate, Liquid, Powder
• Applications: Rt-PCR, Cdna Synthesis, Second Generation Sequenci
• Model NO.: R662201
• Specification: 50 Preps/Kit
• Trademark: Magen Biotech
• Origin: China

Product Description

Product Description

Introduction
This product supplies a simple and rapid extraction of total RNA from tissue and culture cells samples. The kit is based on superparamagnetic particles purification technology, no phenol-chloroform extraction or alcohol precipitation.


The whole extraction processis only 60 minutes. Purified RNA is ready for downstream applications such as RT-PCR, virus RNA testing and so on. MagPure LQ Kit buffers can be used for both manual extraction process and automatic nucleic acid extraction machines.




Specifications

Features	Specifications
Main Functions	Isolation total RNA from tissue and cells and plant
Applications	RT-PCR, cDNA synthesis, second generation sequencing
Purification method	Polydisperse magnetic beads
Purification technology	Magnetic beads technology
Process method	Manual or automatic
Adaptive instrument	Nucleic acid extractor, pipetting workstation
Sample type	Animal tissue, human tissue, cultured cells, lymphocytes, ordinary plant tissue
Sample amount	Cells: 1 x 10^7 Animal tissue: ≤20mg Plant tissue: ≤100mg Yeast cell: ≤5 x 10^6
Yield	5-100μg

 

Principle

The Kit combines the speed and efficiency of silica-based technology with the convenient handling of magnetic particles for purification of total RNA. Samples are lysed and RNA is purified from lysates in one step through its binding to the silica surface of the particles in the presence of a chaotropic salt.


The particles are separated from the lysates using a magnet and DNA is removed by treatment with RNase-free DNase. The magnetic particles are efficiently washed, and RNA is eluted in RNase-free water.




Advantages

• High purity - OD 260 / 280 :1.9-2.0, OD 260 / 230 :1.5-2.0
• Economy - less than 50% of the price of Qiagen and other imported products
• Automatic - extraction without manual participation, saving time and effort
• Universal - suitable for various animal tissues, cultured cells and conventional plant fungal tissues

Kit Contents

Contents	R662201	R662202	R662203
Purification Times	48 Preps	96 Preps	5 x 96 Preps
MagPure RNA Particles	1.7 ml	4.0 ml	18 ml
Proteinase K	24 mg	48 mg	240 mg
Protease Dissolve Buffer	1.8 ml	5 ml	15 ml
DNase I	600 μl	2 x 600 μl	10 x 600 μl
DNase Buffer	30 ml	2 x 30 ml	200 ml
Buffer RTL	40 ml	80 ml	400 ml
Buffer MCB	18 ml	30 ml	150 ml
Buffer MW1	44 ml	66 ml	2 x 220 ml
Buffer RW2	20 ml	50 ml	2 x 100 ml
RNase Free Water	10 ml	30 ml	120 ml

Storage and Stability
MagPure Particles, DNase I and Proteinase K should be stored at 2-8°C upon arrival. However, short-term storage (up to 12 weeks) at room temperature (15-25°C) does not affect their performance.


The remaining kit components can be stored dry at room temperature (15-25°C) and are stable for at least 18 months under these conditions.





 

Packaging & Shipping

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