Hipure Tissue DNA Mini Kit Extraction of DNA From 1~20mg Tissue

• Material: Plate,Liquid,Powder
• Transport Package: Box
• Specification: 50 Preps/Kit
• Trademark: Magen Biotech
• Origin: China
• Model NO.: D3121
• HS Code: 3822009090

Product Description

 

Product Description

Introduction
This product provides fast and easy methods for purification of total DNA for reliable PCR and Southern blotting. 
Total DNA (e.g., genomic, viral, mitochondrial) can be purified from tissue and culture cells.




Specifications

Features	Specifications
Main Functions	Isolation total DNA from 30 mg Tissue, Culture cells, FTA Card
Applications	PCR, southern bolt and virus detection, etc.
Purification method	Mini spin column
Purification technology	Silica technology
Process method	Manual (centrifugation or vacuum)
Sample type	Animal tissue or cultured cells
Sample amount	Animal tissue : 1~20mg Cultured cells : <5 x 10^6
Elution volume	30μl
Time per run	30 - 60 minutes
Liquid carrying volume per column	800µl
Binding yield of column	100µg

Principle
This product is based on silica Column purification. The sample is lysed and digested with lysate and protease, DNA is released into the lysate. Transfer to an adsorption column.

Nucleic acid is adsorbed on the membrane, while protein is not adsorbed and is removed with filtration. After washing proteins and other impurities, Nucleic acid was finally eluted with low-salt buffer (10mm Tris, pH9.0, 0.5mm EDTA).



Advantages
Good repeatability - suitable for extracting high-yield DNA from different types of tissue samples
High purity - for downstream applications such as multiplex and quantitative PCR
Fast - simplified process, extract several samples in 20 minutes (after digestion)
Safety - no phe nol   or chlo roform extraction, no alcohol precipitation



Kit Contents

Contents	D312102	D312103
Purification Times	50	250
HiPure gDNA Mini Columns	50	2 x 125
2ml Collection Tubes	100	5 x 100
Buffer ATL	15 ml	65 ml
Buffer DL	15 ml	65 ml
Buffer GW1	22 ml	110 ml
Buffer GW2	12 ml	50 ml
RNase A	10 mg	50 mg
Proteinase K	24 mg	120 mg
Protease Dissolve Buffer	5 ml	15 ml
Buffer AE	15 ml	60 ml

Storage and Stability
This kit is shipped and stored at room temperature and is valid for 12months.





 

Detailed Photos

Packaging & Shipping

 

Certifications

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