Magpure Plasmid Ef Mini Kit

• Material: Plate,Liquid,Powder
• Transport Package: Box
• Specification: 100 Preps/Kit
• Trademark: Magen Biotech
• Origin: China
• Model NO.: P181402

Product Description

Product Description

Introduction
The MagPure Plasmid purification system uses the paramagnetic bead technology for high-throughput preparation of high-copy or low-copy plasmid DNA from E. coli cells. This kit also can be used with fosmid and BAC vector-based constructs.


The system uses alkaline lysis followed by a MagPure purification to differentially bind plasmid DNA to paramagnetic beads. While the DNA is bound to the beads, contaminants can be rinsed away using a simple washing procedure. Because MagPure uses magnetic separation technology, the protocol does not require vacuum filtration. This makes kit extremely amenable to automation. Plasmid DNA purified with this system is most commonly used in Sanger Sequencing and PCR amplification.





Specifications

Features	Specifications
Main Functions	Isolation up to 20μg endotoxin free plasmid DNA from 1.5-5ml bacterial culture
Applications	Enzyme digestion, sequencing, PCR and labeling,  etc.
Purification technology	Magnetic beads technology
Process method	Manual or automatic
Sample type	Conventional plasmid, plasmid less than 30KB
Sample amount	1.5-5ml
Elution volume	≥50μl
Time per run	≤80 minutes

Principle
This product is based on the purification method of high binding magnetic particles. The sample is lysed and digested under the action of lysate and Lysozyme. DNA is released into the lysate. After adding magnetic particles and binding solution, DNA will be adsorbed on the surface of magnetic particles, and impurities such as proteins will be removed without adsorption.


The adsorbed particles were washed with washing solution to remove proteins and impurities, washed with ethanol to remove salts, and finally DNA was eluted by Elution Buffer.





Advantages

• High purity - purified plasmid can be directly used in sequencing, enzyme digestion and PCR, etc.
• Fast - it takes only 80 minutes to complete the isolation
• High yield - up to 20μg plasmid can be binded in one column

Kit contents

Contents	P181402	P181403	P181404
Purification Times	100 Preps	500 Preps	5000 Preps
RNase A	10 mg	50 mg	2 x 250 mg
Buffer P1	30 ml	150 ml	2 x 800 ml
Buffer P2	30 ml	150 ml	2 x 800 ml
Buffer LEN3	20 ml	80 ml	800 ml
Buffer LN4	60 ml	300 ml	3 x 900 ml
Buffer LN5	60 ml	300 ml	3 x 900 ml
Buffer GW1	53 ml	352 ml	6 x 440 ml
Bind Bead N	4.8 ml	24 ml	3 x 72 ml

Storage and stability
RNase A and Bind Bead N should be stored at 2-8°C upon arrival. However, short-term storage (up to 12 weeks) at room temperature (15-25°C) does not affect its performance. The remaining kit components can be stored dry at room temperature (15-25°C) and are stable for at least 18 months under these conditions.


The entire kit can bestored at 2-8°C, but in this case buffers should be redissolved before use. Make sure that all buffers are at room temperature when used. If any precipitates form in the buffers, warm at 37ºC to dissolve. After addition of RNase A, Buffer P1 is stable for 6 months when stored at 2-8°C.

 

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