HiPure Blood DNA Mini Kit Isolation total DNA 200μl whole blood

• Customization: Available
• CAS No.: /
• Formula: /
• Model NO.: D311102 / D311103
• EINECS: /
• Classification: Biochemical Reagents
• Grade: Reagent Grade
• Specific Usage: For Biological Purpose, For Microbiological, Isolation total DNA from 200ul Whole Blood
• Content: Standard
• Usage: Laboratory Reagents, Analytical Reagents, Diagnostic Reagents, Teaching Reagents, PCR, qPCR, southern bolt and virus detection
• Source: Lab
• Habit Appellation: HiPure Blood DNA Mini Kit
• Application: Industry, Scientific Research, Health, Environmental Protection, Agriculture
• Property: Biochemical Reagent
• Main Functions: Isolation total DNA from 200ul Whole Blood
• Applications: PCR, southern bolt and virus detection, etc
• Purification Method: Mini Spin Column
• Purification Technology: Silica Technology
• Process Method: Manual (Centrifugation or Vacuum)
• Sample Type1: Whole Blood (fresh or frozen), serum, plasma
• Sample amount1: <200μl whole blood or other liquid samples
• Sample amount2: <5*106 lymphocytes or Culture Cells
• Sample amount3: red blood cells:5~20μl whole blood at a time
• Sample Type2: milk, saliva, and other liquid samples
• Elution Volume: ≥20μl
• Time per run: ≤30 minutes
• Liquid Carrying Volume Per Column: 800μl
• Binding Yield of Column: 100μg
• Sample Type3: cultured cells
• Transport Package: Bottle/ Box
• Specification: 50 preps / 250 preps
• Trademark: Magen Biotech
• Origin: China
• HS Code: 3822190090
• Production Capacity: 100000 Per Month

Product Description

Product Description

HiPure Blood DNA Mini Kit

CAT NO	PRODUCT NAME	SIZE	PRICE
D311102	HiPure Blood DNA Mini Kit	50 preps	$99.00
D311103	HiPure Blood DNA Mini Kit	250 preps	$472.00

Blood samples contain rich DNA, including mitochondrial DNA, genomic DNA, circulating DNA (mostly released into blood after tumor cell apoptosis) in white blood cells, as well as parasitic viral or microbial DNA. These DNA are important parameters in clinical testing or diagnosis, which are also valuable materials for medical research. There are three main issues with extracting DNA from blood samples:
1. The sample is highly infectious, posing great harm to operators and the environment.
2. The source of DNA is complex and aportion of the nucleic acid, such as viral DNA or free DNA, may be lost during the operation, leading to downstream detection failure;
3. Blood sample contains a large amount of impurities and inhibitory factors.
Currently there are many methods available for extracting DNA from whole blood samples, such as phenol chloroform extraction, salting out method, etc. However, these methods require pre-treatment of blood sample, which removes red blood cells and isolate white blood cells in the first step. Due to the requirement that it cannot inactivate or kill pathogens during the process of removing red blood cells, the waste liquid (red blood cell lysate) and consumables may be contaminated by pathogens and become infectious, posing a danger to the entire laboratory environment and operators. In addition, during the process of removing red blood cells, useful nucleic acid information such as viruses, microorganisms, or circulating DNA is also lost, leading to experiment or detection failures.
The HiPure Blood DNA Kits series provided by Magen Company uses silica gel column purification technology, which can directly lyse whole blood samples without the need for white blood cell separation. Whole blood samples are directly mixed with lysates and proteases, resulting in the inactivation of pathogens, greatly reducing the infectivity, environmental pollution, and the chance of operators being infected. Due to the direct lysis and digestion of samples, except lymphocyte DNA, other circulating DNA as well as DNA from viruses and microorganisms, can also be recovered.

This product provides fast and easy methods for purification of total DNA for reliable PCR and Southern blotting. Total DNA(e.g., genomic, viral, mitochondrial) can be purified from whole blood (fresh or frozen), plasma, serum, buffy coat, bone marrow, other body fluids, lymphocytes, cultured cells.



Specifications
 

Features	Specifications
Main Functions	Isolation total DNA from 200ul Whole Blood
Applications	PCR, southern bolt and virus detection, etc
Purification method	Mini spin column
Purification technology	Silica technology
Process method	Manual (centrifugation or vacuum)
Sample type	Whole Blood (fresh or frozen), serum, plasma, milk, saliva, and other liquid samples and cultured cells
Sample amount	<200μl whole blood or other liquid samples, <5*106 lymphocytes or Culture Cells Non mammalian animals that have nucleus in red blood cells (rich in DNA, such as birds and fish) : 5~20μl whole blood at a time
Elution volume	≥20μl
Time per run	≤30 minutes
Liquid carrying volume per column	800µl
Binding yield of column	100µg

Principle

This product is based on silica Column purification. The sample is lysed and digested with lysate and protease, DNA is released into the lysate. Transfer to an adsorption column. Nucleic acid is adsorbed on the membrane, while protein is not adsorbed and is removed with filtration. After washing proteins and other impurities, Nucleic acid was finally eluted with low-salt buffer (10mm Tris, pH9.0, 0.5mm EDTA).

Advantages

• High quality DNA - meet a variety of downstream applications, including PCR, qPCR, enzyme digestion, hybridization, etc.
  Fast - without separation of leukocytes, organic extraction or ethanol precipitation
  Simple - all nucleic acids can be obtained by direct digestion
  Wide applicability- handle a variety of liquid samples

Kit Contents

 

Contents	D311102	D311103
Purification Times	50	250
HiPure DNA Mini Columns I	50	2 x 125
2ml Collection Tubes	100	5 x 100
Buffer AL	15 ml	60 ml
Buffer DW1	30 ml	150 ml
Buffer GW2*	12 ml	50 ml
Proteinase K	24 mg	120 mg
Protease Dissolve Buffer	1.8 ml	10 ml
Buffer AE	15 ml	60 ml

Storage and Stability

Proteinase K should be stored at 2-8°C upon arrival. However, short-term storage (up to 12 weeks) at room temperature (15-25°C) does not affect their performance. The remaining kit components can be stored at room temperature (15-25°C) and are stable for at least 18 months under these conditions.


Purchase Guide
 

Name	CAT NO	Sample amount	Leukocyte protocol*	Colum type	Elutio volume	Average yield
HiPure Blood DNA Mini Kit	D3111	10-200μl	1ml	2ml column	≥20μl	5-9μg/200μl
HiPure Tissue&Blood DNA Midi Kit	D3113	0.2-2ml	10ml	1.5ml column	≥300μl	20-40μg/1m
HiPure Tissue&Blood DNA Maxi Kit	D3115	3 -10ml	10ml	15ml column	≥700μl	20-40μg/1m
HiPure Tissue&Blood DNA 96 Kit	D3117	1-200μl	1ml	96 well plate		3-8μg/200μl

*Note:Leukocyte protocol can be used when large volume whole blood samples need to be processed. Whole blood was treated with red blood cell lysate, and white blood cells were obtained by centrifugation before extraction


 

Detailed Photos

 

 

 

 

Test Report

 

 

Company Profile

 

 

FAQ

 

Q1.What is the Trade Term?
A1:Ex-work factory (Guangzhou)

Q2. How long is the guarantee (period)?
A2:The product can be stored properly for at least 18 months .

Q3.Are you a manufacturer ? 
A3:Yes, we were established in 2012 and our headquarter is in Guangzhou. All products can be provided with OEM packaging, greatly reduce the cost, and can be flexible in technology and other aspects of communication and cooperation.

Q4.How long is our Production leading time?
A4:Generally our lead time is 2-7 working days after receiving 100% prepayment. If order in bulk , lead time will be longer. Customized bulk quantities are determined by the specific quantity.

Q5.What is the Payment term?
A5:T/T, Paypal, Alipay, Western Union. 100% payment in advance.

Q6.What is the packing details?
A6:The outer packaging of general products is cardboard boxes. Low-temperature products will have ice packs places in foam boxes and then packed into cartons.

Q7. What support you will have to assemble this extractor?
A7: Our extraction instrument is integrated, easy to operate and does not require assembly.

Q8.What kind of documents we will provide to you?
A8:B/L or AWB number, Commercial Invoice, Packing List, MSDS, COA.

Q9. How to apply the free samples?
A9: For free samples we have to know your company name, your E-mail, your FedEx account, and the detail address of your company include the phone number. You can contact with us to apply the free samples.

Q10. Do you accept the small orders?
A10: Yes, we accept the small orders. We will try our best to support our customer's business whether the small trail order.