Product Description
HiPure Viral RNA Kit
CAT NO |
PRODUCT NAME |
SIZE |
PRICE |
R417102 |
HiPure Viral RNA Kit |
50 preps |
$99.00 |
R417103 |
HiPure Viral RNA Kit |
250 preps |
$448.00 |
△ Introduction
Hipure viral RNA kit is suitable for purifying viral RNA from samples such as cell-free body fluid or culture medium. The kit is based on silica gel column purification technology. It requires no toxic phenol chloroform extraction and time-consuming alcohol precipitation in the extraction. The whole extraction process takes only 25 minutes. The kit is suitable for extracting viral RNA from 1-140µl cell-free fluid samples such as serum, plasma, cell-free body fluid or culture medium. The product has successfully extracted hepatitis B A/C, hepatitis C RNA, SARS and HIV. The obtained RNA can be directly used for RT-PCR, Northern hybridization and virus detection.
△ Details
Features |
Specifications |
Main Functions |
Extract viral RNA from 140μl cell-free samples |
Applications |
RT-PCR, Northern hybridization, and various virus detection |
Purification method |
Mini spin column |
Purification technology |
Silica technology |
Process method |
Manual (centrifugation or vacuum) |
Sample type |
Cell-free body fluid or culture medium |
Sample amount |
140μl |
Elution volume |
≥15μ |
Time per run |
≤25 minutes |
Liquid carrying volume per column |
800μl |
Binding yield of column |
100μg |
△ Advantages
- Fast - several samples can be extracted in 20 minutes by column method
- High quality - high purity total RNA can be directly used in various sensitive downstream applications
- Safe - no phenol chloroform extraction required
- Sensitive - RNA can be recovered at the level of PG
- High yield - carrier RNA contained in the product maximize the recovery of trace nucleic acid
△ Kit Contents
Contents |
R417102 |
R417103 |
Purification Times |
50 Preps |
250 Preps |
HiPure Viral Micro Columns |
50 |
250 |
2ml Collection Tubes |
100 |
500 |
Buffer VRL |
50 ml |
200 ml |
Carrier RNA |
310 µg |
3 x 310 µg |
Buffer VHB* |
13 ml |
110 ml |
Buffer RW2* |
20 ml |
2 x 50 ml |
Nuclease Free Water |
10 ml |
30 ml |
△ Storage and Stability
Carrier RNA should be stored at 2-8°C upon arrival. However, short-term storage (up to 12 weeks) at room temperature (15-25°C) does not affect their performance. The remaining kit components can be stored at room temperature (15-25°C) and are stable for at least 18 months under these conditions.
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