Hipure Yeast DNA Kit Isolation Total DNA From Yeast Cultures

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Number of Employees
4
Year of Establishment
2021-01-29
  • Hipure Yeast DNA Kit Isolation Total DNA From Yeast Cultures
  • Hipure Yeast DNA Kit Isolation Total DNA From Yeast Cultures
  • Hipure Yeast DNA Kit Isolation Total DNA From Yeast Cultures
  • Hipure Yeast DNA Kit Isolation Total DNA From Yeast Cultures
  • Hipure Yeast DNA Kit Isolation Total DNA From Yeast Cultures
  • Hipure Yeast DNA Kit Isolation Total DNA From Yeast Cultures
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Overview

Basic Info.

Model NO.
D314702 / D314703
EINECS
/
Classification
Biochemical Reagents
Grade
Reagent Grade
Specific Usage
For Biological Purpose, For Microbiological, DNA Isolation From Yeast Samples
Content
Standard
Usage
Laboratory Reagents, Analytical Reagents, Diagnostic Reagents, Teaching Reagents, PCR, Southern Blot, Enzyme Digestion and Ngs
Source
Lab Production
Habit Appellation
Hipure Yeast DNA Kit
Application
Industry, Scientific Research, Health, Environmental Protection, Agriculture, PCR, Southern Blot and Enzyme Digestion
Property
Biochemical Reagent
Main Functions
Isolation Total DNA From Yeast Cultures
Applications1
PCR, Southern Blot and Enzyme Digestion, etc.
Purification Method
Mini Spin Column
Purification Technology
Silica Technology
Process Method
Manual (Centrifugation or Vacuum)
Sample Type
Yeast Culture
Sample Amount
Bacterial Culture: 1-1.5 Ml
Elution Volume
≥30μl
Time Per Run
≤60 Minutes
Liquid Carrying Volume Per Column
800μl
Binding Yield of Column
100μg
Total DNA
Yeast (<5X 107)
Sample
Free Sample
Transport Package
Bottle/ Box
Specification
50 preps / 250 preps
Trademark
Magen Biotech
Origin
China
Production Capacity
100000 Per Month

Product Description

Product Description

HiPure Yeast DNA Kit

CAT NO PRODUCT NAME SIZE PRICE
D314702 HiPure Yeast DNA Kit 50 preps $154.00
D314703 HiPure Yeast DNA Kit 250 preps $694.00








△ Introduction

This product provides a reliable solution for DNA isolation from yeast samples. Total DNA can be purified from yeast (<5x 107) without phenol or chloroform. The whole extraction can be finished within 60 minutes. Purified DNA can be directly used for PCR, Southern blot, ect.






△ Details
Features Specifications
Main Functions Isolation total DNA from yeast cultures
Applications PCR, southern blot and enzyme digestion, etc.
Purification method Mini spin column
Purification technology Silica technology
Process method Manual (centrifugation or vacuum)
Sample type Yeast culture
Sample amount

Bacterial culture: 1-1.5 ml

Elution volume ≥30μl
Time per run ≤60 minutes
Liquid carrying volume per column 800μl
Binding yield of column 100μg




△ Principle

This product is based on silica Column purification. The sample is lysed and digested with lysate and protease, DNA is released into the lysate. Transfer to an adsorption column. Nucleic acid is adsorbed on the membrane, while protein is not adsorbed and is removed with filtration. After washing proteins and other impurities, Nucleic acid was finally eluted with low-salt buffer (10mm Tris,pH9.0, 0.5mm EDTA).






△  Advantages
  • Fast - several samples can be extracted in 40 minutes (after digestion)
  • High purity - purified DNA can be directly used in various downstream applications
  • Good repeatability - silica technology can obtain ideal results every time
  • High recovery - DNA can be recovered at the level of PG
  • Sufficient components - lysozyme, protease K, RNase A and glass beads






△ Kit Contents
Contents D314702 D314703
Purification Times 50 Preps 250 Preps
Hipure DNA Mini Columns I 50 250
2ml Collection Tubes 50 250
Glass Beads (0.4~0.6mm) 20 g 90 g
Buffer SE 30 ml 150 ml
Lyticase 1.8 ml 5 x 1.8 ml
Buffer ATL 30 ml 150 ml
ReagentDX 500 μl 1500 μl
Buffer DL 30 ml 150 ml
Buffer GW1* 13 ml 66 ml
Buffer GW2* 20 ml 2 x 50 ml
Proteinase K 12 mg 60 mg
Protease Dissolve Buffer 1.8 ml 5 ml
Buffer AE 15 ml 30 ml







△ Storage and Stability

Proteinase K should be stored at 2-8°C upon arrival. However, short-term storage (up to 12 weeks) at room temperature (15-25°C) does not affect their performance. The remaining kit components can be stored dry at room temperature (15-25°C) and are stable for at least 18 months under these conditions. Buffer ATL may precipitate at low temperature. Dissolve it by 37ºC water bath.



 

Company Profile

Hipure Yeast DNA Kit Isolation Total DNA From Yeast CulturesHipure Yeast DNA Kit Isolation Total DNA From Yeast CulturesHipure Yeast DNA Kit Isolation Total DNA From Yeast CulturesHipure Yeast DNA Kit Isolation Total DNA From Yeast Cultures

 

Exhibition all over the world

Hipure Yeast DNA Kit Isolation Total DNA From Yeast CulturesHipure Yeast DNA Kit Isolation Total DNA From Yeast Cultures

 

Packaging & Shipping

 

Hipure Yeast DNA Kit Isolation Total DNA From Yeast CulturesHipure Yeast DNA Kit Isolation Total DNA From Yeast CulturesHipure Yeast DNA Kit Isolation Total DNA From Yeast CulturesHipure Yeast DNA Kit Isolation Total DNA From Yeast CulturesHipure Yeast DNA Kit Isolation Total DNA From Yeast Cultures





 

Certifications

Hipure Yeast DNA Kit Isolation Total DNA From Yeast CulturesHipure Yeast DNA Kit Isolation Total DNA From Yeast CulturesHipure Yeast DNA Kit Isolation Total DNA From Yeast Cultures



 

Laboratory

Hipure Yeast DNA Kit Isolation Total DNA From Yeast Cultures

 

Workshop

Hipure Yeast DNA Kit Isolation Total DNA From Yeast Cultures
 

Fininshed product area

Hipure Yeast DNA Kit Isolation Total DNA From Yeast Cultures

Hipure Yeast DNA Kit Isolation Total DNA From Yeast Cultures
 

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