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HiPure Yeast DNA Kit
CAT NO | PRODUCT NAME | SIZE | PRICE |
D314702 | HiPure Yeast DNA Kit | 50 preps | $154.00 |
D314703 | HiPure Yeast DNA Kit | 250 preps | $694.00 |
△ Introduction
Features | Specifications |
Main Functions | Isolation total DNA from yeast cultures |
Applications | PCR, southern blot and enzyme digestion, etc. |
Purification method | Mini spin column |
Purification technology | Silica technology |
Process method | Manual (centrifugation or vacuum) |
Sample type | Yeast culture |
Sample amount |
Bacterial culture: 1-1.5 ml |
Elution volume | ≥30μl |
Time per run | ≤60 minutes |
Liquid carrying volume per column | 800μl |
Binding yield of column | 100μg |
Contents | D314702 | D314703 |
Purification Times | 50 Preps | 250 Preps |
Hipure DNA Mini Columns I | 50 | 250 |
2ml Collection Tubes | 50 | 250 |
Glass Beads (0.4~0.6mm) | 20 g | 90 g |
Buffer SE | 30 ml | 150 ml |
Lyticase | 1.8 ml | 5 x 1.8 ml |
Buffer ATL | 30 ml | 150 ml |
ReagentDX | 500 μl | 1500 μl |
Buffer DL | 30 ml | 150 ml |
Buffer GW1* | 13 ml | 66 ml |
Buffer GW2* | 20 ml | 2 x 50 ml |
Proteinase K | 12 mg | 60 mg |
Protease Dissolve Buffer | 1.8 ml | 5 ml |
Buffer AE | 15 ml | 30 ml |
△ Storage and Stability
Proteinase K should be stored at 2-8°C upon arrival. However, short-term storage (up to 12 weeks) at room temperature (15-25°C) does not affect their performance. The remaining kit components can be stored dry at room temperature (15-25°C) and are stable for at least 18 months under these conditions. Buffer ATL may precipitate at low temperature. Dissolve it by 37ºC water bath.