HiPure Total RNA Kit spin columns binding Applications for RT-PCR and qRT-PCR

• Customization: Available
• CAS No.: /
• Formula: /
• Model NO.: R401102 / R401103
• EINECS: /
• Classification: Biochemical Reagents
• Grade: Reagent Grade
• Specific Usage: For Biological Purpose, For Microbiological, Isolation total RNA (not include miRNA)
• Content: Standard
• Usage: Laboratory Reagents, Analytical Reagents, Diagnostic Reagents, Teaching Reagents, PCR, qPCR, southern bolt and virus detection
• Source: Lab
• Habit Appellation: HiPure Total RNA Kit
• Application: Industry, Scientific Research, Health, Environmental Protection, Agriculture
• Property: Biochemical Reagent
• Main Functions: Isolation total RNA(not include miRNA)
• Applications: RT-PCR, qRT-PCR, Northern hybridization
• Applications2: second generation sequencing
• Purification Method: Mini Spin Column
• Purification Technology: Silica Technology
• Process Method: Manual (Centrifugation or Vacuum)
• Sample Type1: Animal soft tissue, cultured cells
• Sample Type2: lymphocytes, simple plant tissue
• Sample Amount: Cells: ≤1 x 107 Animal tissue:1-20mg
• Sample amount1: Plant leaves: 50-150 mg, Yeast cells: 5 x 106
• Yield: 2-100μg
• Elution Volume: ≥50μl
• Time per run: ≤15minutes(1-24 samples)
• Liquid Carrying Volume Per Column: 800μl
• Binding Yield of Column: 100μg
• Transport Package: Bottle/ Box
• Specification: 50 preps / 250 preps
• Trademark: Magen Biotech
• Origin: China
• HS Code: 3822190090
• Production Capacity: 100000 Per Month

Product Description

Product Description

HiPure Total RNA Kit
 

CAT NO	PRODUCT NAME	SIZE	PRICE
R401102	HiPure Total RNA Kit	50 preps	$99.00
R401103	HiPure Total RNA Kit	250 preps	$448.00

The HiPure Total RNA Kit provides fast purification of high-quality RNA from cells, tissues, and yeast using silica-membrane spin columns with a binding capacity of 100μg RNA. There is no need for phenol/chloroform extractions and time-consuming steps such as CsCl gradient ultracentrifugation or isopropanol precipitation. RNA purified using the HiPure Total RNA Purification System can be used for applications such as RT-PCR, Northern blotting, poly A+ RNA (mRNA) purification, nuclease protection, and in vitro translation.


Specifications

Features	Specifications
Main Functions	Isolation total RNA (not include miRNA) from animal tissues, cells and simple plant tissues using one column
Applications	RT-PCR, qRT-PCR, Northern hybridization, second generation sequencing
Purification method	Mini spin column
Purification technology	Silica technology
Process method	Manual (centrifugation or vacuum)
Sample type	Animal soft tissue, cultured cells, lymphocytes, simple plant tissue
Sample amount	Cells: ≤1 x 107 Animal tissue: 1-20 mg Plant leaves: 50-150 mg Yeast cells: 5 x 106
Yield	2-100μg
Elution volume	≥50μl
Time per run	≤15 minutes(1-24 samples)
Liquid carrying volume per column	800µl
Binding yield of column	100µg

Principle
HiPure RNA technology simplifies total RNA isolation. Samples are first lysed and then homogenized. Ethanol is added to the lysate to provide ideal binding conditions. The lysate is then loaded onto the HiPure silica membrane and RNA binds to the silica membrane, and all contaminants are efficiently washed away. For certain RNA applications that are sensitive to very small amounts of DNA, the residual amounts of DNA remaining can be removed using a convenient on-column DNase treatment. Pure, concentrated RNA is eluted in water.

Advantages
Efficient removal of DNA - unique genomic DNA removal column without DNase treatment
High quality - high purity total RNA can be directly used in various sensitive downstream applications
Fast - several samples can be extracted in 20 minutes by column method
Safe - no phenol chloroform extraction required
Sensitive - RNA can be recovered at the level of PG

Kit Contents

Contents	R401102	R401103
Purification Times	50 Preps	250 Preps
HiPure RNA Mini Columns	50	250
2ml Collection Tubes	50	250
RTL Lysis Buffer	50 ml	200 ml
RNA Binding Buffer	15 ml	75 ml
Buffer RW1	50 ml	200 ml
Buffer RW2*	20 ml	2 x 50 ml
RNase Free Water	10 ml	30 ml

Storage and Stability
The Kit can be stored dry at room temperature (15-25°C) and are stable for at least 18 months under these conditions.

 

Detailed Photos

 

Protocol

 

 

Test Report

 

 

Company Profile

 

 

FAQ

 

Q1.What is the Trade Term?
A1:Ex-work factory (Guangzhou)

Q2. How long is the guarantee (period)?
A2:The product can be stored properly for at least 18 months .

Q3.Are you a manufacturer ? 
A3:Yes, we were established in 2012 and our headquarter is in Guangzhou. All products can be provided with OEM packaging, greatly reduce the cost, and can be flexible in technology and other aspects of communication and cooperation.

Q4.How long is our Production leading time?
A4:Generally our lead time is 2-7 working days after receiving 100% prepayment. If order in bulk , lead time will be longer. Customized bulk quantities are determined by the specific quantity.

Q5.What is the Payment term?
A5:T/T, Paypal, Alipay, Western Union. 100% payment in advance.

Q6.What is the packing details?
A6:The outer packaging of general products is cardboard boxes. Low-temperature products will have ice packs places in foam boxes and then packed into cartons.

Q7. What support you will have to assemble this extractor?
A7: Our extraction instrument is integrated, easy to operate and does not require assembly.

Q8.What kind of documents we will provide to you?
A8:B/L or AWB number, Commercial Invoice, Packing List, MSDS, COA.

Q9. How to apply the free samples?
A9: For free samples we have to know your company name, your E-mail, your FedEx account, and the detail address of your company include the phone number. You can contact with us to apply the free samples.

Q10. Do you accept the small orders?
A10: Yes, we accept the small orders. We will try our best to support our customer's business whether the small trail order.