Hipure Circulating DNA MIDI Kit C vacuum Protocol

• CAS No.: /
• Formula: /
• EINECS: /
• Classification: Biochemical Reagents
• Grade: Classic
• Specific Usage: For Biological Purpose, For Microbiological, For Refractive Index, Isolation Circulating DNA From 1-5ml Plasma, Serum
• Model NO.: D3182C
• Content: Standard
• Usage: Laboratory Reagents, Diagnostic Reagents, Teaching Reagents, Qpcr, Liquid or Solid Chip Analysis, Hybridization
• Source: Laboratory Production
• Habit Appellation: Circulating DNA MIDI Kit C (Vaccum Protocol
• Application: Industry, Scientific Research, Health, Environmental Protection, Agriculture, Isolation Circulating DNA From 1-5ml Plasma, Serum
• Product Name: Hipure Circulating DNA MIDI Kit C (Vaccum Protocol
• Applications: Qpcr, Liquid or Solid Chip Analysis, Hybridization
• Purification Method: Mini Spin Column
• Purification Technology: Silica Technology
• Process Method: Manual (Vacuum)
• Sample Type: Serum, Plasma and Other Cell-Free Fluid Samples
• Sample Amount: 1-5ml
• Elution Volume: ≥50μl
• Time Per Run: ≤60 Minutes
• Liquid Carrying Volume Per Column: 4ml
• Binding Yield of Column: 1mg
• Sample: Free Sample
• After-Sales Service: Technical Support
• Shipping: FedEx, DHL, Door to Door
• Transport Package: Box
• Specification: 10 preps/ 50 preps
• Trademark: Magen Biotech
• Origin: China
• HS Code: 90279000
• Production Capacity: 100000 Per Month

Product Description

Product Description

HiPure Circulating DNA Midi Kit C (Vaccum Protocol)

CAT NO	PRODUCT NAME	SIZE	PRICE
D318201C	HiPure Circulating DNA Midi Kit C	10 preps	$136.00
D318202C	HiPure Circulating DNA Midi Kit C	50 preps	$547.00

△ Introduction

Free-circulating nucleic acids, such as tumor-specific extracellular DNA fragments and mRNAs in the blood or fetal nucleic acids in maternal blood, are present in serum or plasma usually as short fragments, <1000bp (DNA). HiPure Circulating DNA Midi Kit enables efficient purification of these circulating nucleic acids from human plasma, serum, or urine. The extracted products can be used for clinical in vitro detection.


△ Details

Features	Specifications
Main Functions	Isolation circulating DNA from 1-5ml plasma, serum, body fluids using vacuum protocol
Applications	qPCR, liquid or solid chip analysis, hybridization and SNP detection, etc.
Purification method	Mini spin column
Purification technology	Silica technology
Process method	Manual (vacuum)
Sample type	Serum, plasma and other cell-free fluid samples
Sample amount	1-5ml
Elution volume	≥50μl
Time per run	≤60 minutes
Liquid carrying volume per column	4ml
Binding yield of column	1mg

△ Advantages

• High yield - most optimal process, free DNA (>50bp) can be obtained to the maximum extent
• High concentration - low elution volume, ensuring high nucleic acid concentration
• High purity - low alcohol binding method, completely removing inhibitor and protein pollution
• High recovery - DNA can be recovered at thelevel of PG by silica gel column purification

△ Kit Contents
 

Contents	D318201C	D318202C
Purification Times	10	50
Buffer ACL	50 ml	250 ml
Buffer ACB*	60 ml	300 ml
Buffer DCW1*	4.4 ml	22 ml
Buffer DCW2*	5 ml	10 ml
Proteinase K	110 ml	540 mg
Protease Dissolve Buffer	10 ml	30 ml
Carrier RNA	110 μg	110 μg
Nuclease Free Water	10 ml	20 ml
HiPure CFDNA Mini Columns	10	50
2 ml Collection Tubes	20	100
Extender Tube	10	50
Vac-Connector	10	50

△ Storage and Stability

Proteinase K, Carrier RNA should be stored at 2-8°C upon arrival. However, short-term storage (up to 12 weeks) at room temperature (15-25°C) does not affect their performance. The remaining kit components can be stored dry at room temperature (15-25°C) and are stable for at least 18 months under these conditions. The entire kit can be stored at 2-8°C, but in this case buffers should be redissolved before use. Make sure that all buffers are at room temperature when used.


△ Experiment Data

 

Human plasma DNA extraction comparison between Qiagen with Magen






 

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