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Classification: | Biochemical Reagents |
Grade: | Reagent Grade |
Specific Usage: | For Biological Purpose, For Microbiological, For Microscopic Purpose, Isolation Circulating DNA From 1-5ml Plasma, Serum |
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HiPure Circulating DNA Kit
CAT NO | PRODUCT NAME | SIZE | PRICE |
IVD3182 | HiPure Circulating DNA Kit | 50 preps | $547.00 |
△ Introduction
Free-circulating nucleic acids, such as tumor-specific extracellular DNA fragments and mRNAs in the blood or fetal nucleic acids in maternal blood, are present in serum or plasma usually as short fragments, <1000bp (DNA). HiPure Circulating DNA Midi Kit enables efficient purification of these circulating nucleic acids from human plasma, serum, or urine. The extracted products can be used for clinical in vitro detection.
△ Details
Features | Specifications |
Main Functions | Isolation circulating DNA from 1-5ml plasma, serum, body fluids using vacuum protocol |
Applications | qPCR, liquid or solid chip analysis, hybridization and SNP detection, etc. |
Purification method | Mini spin column |
Purification technology | Silica technology |
Process method | Manual (vacuum) |
Sample type | Serum, plasma and other cell-free fluid samples |
Sample amount | 1-5ml |
Elution volume | ≥50μl |
Time per run | ≤60 minutes |
Liquid carrying volume per column | 4ml |
Binding yield of column | 1mg |
△ Advantages
Contents | IVD3182 |
Purification Times | 50 |
Buffer ACL | 250 ml |
Buffer ACB* | 300 ml |
Buffer DCW1* | 22 ml |
Buffer DCW2* | 10 ml |
Proteinase K | 540 mg |
Protease Dissolve Buffer | 30 ml |
Carrier RNA | 110 μg |
Nuclease Free Water | 20 ml |
HiPure CFDNA Mini Columns | 50 |
2 ml Collection Tubes | 100 |
Extender Tube | 50 |
Vac-Connector | 50 |
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