Magpure Viral Nucleic Acid Isolation Kit with Preassembly 96 Well Plate

• Transport Package: Box
• Trademark: Magen Biotech
• Origin: China
• Model NO.: IVD5412-TL-06

Product Description

Product Description

Product Name MagPure Viral Nucleic Acid Isolation Kit
Product specifications IVD5412--TL-06,6 x 16 Preps
Intended Use
This kit is used for extracting total viral nucleic acid from non-cell/low cell content biological samples such as body fluid, serum, plasma, immersion solution, tissue homogenate supernatant, culture supernatant, etc., the extracted products can be used for clinical in vitro detection.

Introduction
This product is based on the purification method of high binding magnetic particles. The sample is lysed and digested under the action of lysate and Protease. DNA / RNA is released into the lysate. After adding magnetic particles and binding solution, DNA / RNA will be adsorbed on the surface of magnetic particles, and impurities such as proteins will be removed without adsorption. The adsorbed particles were washed with washing solution to remove proteins and impurities, washed with ethanol to remove salts, and finally DNA/RNA was eluted by Buffer AVE.


Main Composition
 

Cat.No	Prepackage Reagent	IVD5412--TL-06
PK/Carrier RNA		50mg/310ug
Protease Dissolve Buffer Blue		5 ml
Tip Plate	96-Tip and DW Plate	1
Sample Plate (DW Plate)	500µl Buffer MLB, 20µl MagPure Particles N	1
Wash 1 Plate (DW Plate)	500µl Buffer MW1	1
Wash 2 Plate (DW Plate)	500µl Buffer CW	1
Elute Plate (DW Plate)	100µl Buffer AVE	1

Storage conditions and validity
This kit is shipped and stored at room temperature and is valid for 12 months.


Operation of KingFisher Flex

1. Take out the required components of the kit and remove the sealing bag and sealing film.

2. Add 20ul PK/carrier RNA into the hole of Sample Plate.

3. Add 200~300ul Sample into the hole of Sample Plate.

4. Start the corresponding program (IVD5412_F_96P).

5. Finish the operation after ~15 minutes.

6. Remove the 96-well plate and store the product (Elute Plate) at -20~8ºC.

Operation of Auto-Pure 96(ALLSHENG)

1. Take out the required components of the kit and remove the sealing bag and sealing film.

2. Add 20ul PK/carrier RNA into the hole of Sample Plate.

3. Add 200~300ul Sample into the hole of Sample Plate.

4. Plate the Tip Plate and the reagent Plate into the machine.

 

Plate 1	Plate 2	Plate 3	Plate 4	Plate 8
Tip Plate	Sample Plate	Wash 1 Plate	Wash 2 Plate	Elute Plate

1. Start the corresponding program (IVD5412_F_96).
2. Finish the operation after ~18 minutes.
3. Remove the 96-well plate and store the product (Elute Plate) at -20~8ºC.
4. Program Setting at Auto-Pure 96.

 

Step	Name	Plate	Mix Time	Mix Range	Wait time	Volume	Speed	Temp.	Magnet
1	-Load-	1
2	Bind	2	5	80	0	700	6	Close	2
3	Wash 1	3	1	80	0	500	6	Close	1
4	Wash 2	4	1	80	0	500	6	Close	1
5	Dry	5	0	80	1	200	6	Close	0
6	Elute	8	4	80	0	100	6	55	3
7	-Upload-	4

Operation of liquid station(BGI MSP96,Agilent Bravo)

1. Take out the required components of the kit and remove the sealing bag and sealing film.

2. Add 10ul PK/carrier RNA into the hole of 1.2ml 96 Well Plate.

3. Add 300~320µl Buffer MLB/Beads Mix (from Sample Plate) to 1.2ml 96 Well Plate.

4. Add 160µl of the sample to 1.2ml 96-well plate and shake at ~ 1000rpm for 6 minutes.

5. Transfer to the magnetic stand. Stand for ~2 minutes to absorb the magnetic beads. Completely remove and discard the cleared supernatant.

6. Add 160µl Buffer CW (From Wash 2 Plate) and shake at ~1000rpm for 90 seconds. Transfer to a magnetic stand and let it stand for ~1 minutes to attract magnetic beads. Completely remove and discard the cleared supernatant.

7. Repeat step 6 once.

8. Heat at 55~60°C for 6 minutes.

9. Add ~50µl Buffer AVE (From Elute Plate) and shake at ~ 900rpm for 5 minutes.

10. Transfer to a magnetic stand and let stand for 1 minutes.

11.Transfer the DNA / RNA solution to a new 0.5 ml 96-well plate.

Product performance

1. Appearance inspection: The kit should be completely composed, the appearance of the package should be clean, no leakage,     and no damage; the signs and labels should be clear.
 

2. Nucleic acid purity: Extract 1mg liver homogenate (PBS, 200µl) according to the instructions. The OD260 / 280 value is 1.7-2.0, A260 / 230 value is 1.2-1.8, and the CV value is less than 10%.
 

3. Nucleic acid yield: Extract 1mg liver homogenate (PBS, 200µl) according to the instructions, the yield is 2~ 5ug, and the CV value is less than 10%.
 

4.Nucleic acid integrity: 1mg liver homogenate (200µl) was extracted according to the instructions. There was no obvious degradation of DNA / RNA during electrophoresis of the product.

Packaging & Shipping

Certificate

Laboratory

Workshop

Fininshed product area