Magpure Particles N Concentration 70 Mg/Ml 0.2~2um Silica Beads

• CAS No.: 7732-18-5 / 1317-61-9 / 7647-14-5
• Classification: Biochemical Reagents
• Grade: Biological Reagents Grade
• Specific Usage: For Biological Purpose, For Microbiological, For Microscopic Purpose, Plasmid Extraction, Gel DNA Recovery, Viral Rna
• Content: Standard
• Usage: Laboratory Reagents, Analytical Reagents, Diagnostic Reagents, Teaching Reagents, Genomic DNA and Rna Extraction
• Model NO.: C14110/ C14111/ C14112/ C14113
• Source: Chemical Material
• Habit Appellation: Magpure Particles N
• Application: Industry, Scientific Research, Health, Environmental Protection, Agriculture, Gel DNA Recovery, Product Purification
• Property: Biochemical Reagent
• Concentration: 70 Mg/Ml
• Appearance: Suspension of Yellowish Brown Particles
• Surface Functional Group: Si-Oh, Silanol
• Dispersibility: Polydisperse Amorphous
• Particle Size: 0.2-2 μm
• Preservation Conditions: Room Temperature, Valid for up to 2 Years.
• Preservation Conditions2: Store in 2-8°c to Prevent Microbial Growth.
• Magnetic Response Speed: ~60 Seconds
• Settling Velocity: >10 Minutes
• High Salt Mediated Binding: >2m Guanidine Isothiocyanate, DNA Recovery up to 8
• Alcohol Mediated Binding: 2m Guanidine Hydrochloride / Isopropanol (30%)
• Peg8000 Mediated Binding: The Recovery of DNA/Rna Was up to 85%
• DNA Residue: <1 Ppm
• Recommended Application: Plasmid Extraction, Gel DNA Recovery
• Sample: Free for Testing
• Transport Package: Bottle
• Specification: 100ml/ 400ml/ 3x400ml /10x400ml
• Trademark: Magen Biotech
• Origin: China
• HS Code: 2821100000
• Production Capacity: 100000 Per Month

Product Description

Product Description

MagPure Particles N

(Magnetic beads for viral DNA/RNA extraction, plasmid, DNA/RNA Clean Up, 0.2~2um silica beads)
 

CAT NO	PRODUCT NAME	SIZE	PRICE
C14110	MagPure Particles N	100 ml	$599.00
C14111	MagPure Particles N	400 ml	$2,199.00
C14112	MagPure Particles N	3 x 400 ml	$5,999.00
C14113	MagPure Particles N	10 x 400 ml	$15,999.00

△ Introduction

Magnetic bead nucleic acid purification technology uses nano or micron superparamagnetic material as the matrix, generally black ferric oxide or yellowish brown ferricoxide as the magnetic material. The surface of bead is coated with appropriate functional groups, which can adsorb nucleic acid.


Magnetic beads commonly used for nucleic acids, containing carboxyl groups, hydroxyl groups, or silicon groups. Silicon-based magnetic beads are the most common, and its principle of adsorbing nucleic acid is consistent with the classical glass milk purification technology or glass fiber filter membrane purification method.

Magpure particle is a kind of polydisperse fast speed silica magnetic beads.The core is ferricoxide, accounting for 50%, and the surface coating is silica, accounting for 50%. The product can be used for plasmid extraction, gel DNA recovery, product purification, genomic DNA and RNA extraction, and viral nucleic acid extraction.






△ Details

Features	Specifications
Concentration	70 mg/ml
Appearance	Suspension of yellowish brown particles
Surface functional group	Si-OH, Silanol
Dispersibility	Polydisperse Amorphous
Particle size	0.2-2 μm
Preservation conditions	Room Temperature, valid for up to 2 years. It is recommended to store in 2-8°C to prevent microbial growth.
Magnetic response speed	~60 seconds
Settling velocity	>10 minutes
High salt mediated binding	>2M guanidine isothiocyanate, DNA recovery up to 80%
Alcohol mediated binding	2M guanidine hydrochloride / isopropanol (30%), and the recovery of DNA / RNA was as high as 85%
PEG8000 mediated binding	The recovery of DNA/RNA was up to 85%
DNase/RNase	Not detected
DNA residue	<1 ppm
Recommended application	Plasmid extraction, gel DNA recovery, viral nucleic acid isolation

△ Principle

Highsalt mediated binding: in the solution containing 2-4M guanidine isothiocyanate, Magpure particles can selectively recover DNA molecules, and impurities such as protein polysaccharides are not adsorbed.

Alcohol mediated binding: in the solution containing guanidine salt and alcohol (>25%), Magpure particles can selectively recover DNA/RNA molecules, and proteins and other impurities are not adsorbed.


After biological samples are treated with digestive solution or lysis Buffer, DNA/RNA is released from cells, organelles and protein complexes (ribosomes and nucleosomes) into reagents. After Magpure particles and binding solution are added, DNA/RNA is adsorbed to the surface of Magpure particles to form DNA/RNA bead complex. Under the action of the magnetic field, the magnetic beads are separated and collected, and the impurities such as protein are removed with the waste liquid. After two or three steps of further cleaning, the DNA/RNA magnetic bead complex is resuspended in sterilized water or TE buffer, and the DNA/RNA falls off from the surface of the magnetic beads, so as to achieve the purpose of purification.

△ Purchase Guide

Features	MagPure Particles	MagPure Particles N	MagPure Particles G	MagPure Particles F	MagBind Particles
Cat.No.	C1410	C1411	C1412	C1414	C1413
Concentration	100mg/ml	70mg/ml	40mg/ml	50mg/ml	10mg/ml
Form	Amorphous and Porous	Amorphous and Porous	Porous	Amorphous	Nonporous
Surface function	Si-OH, Silica Beads	Si-OH, Silica Beads	Si-OH, Silica Beads	Si-OH, Silica Beads	COOH, Carboxyl Beads
Dispersion	Polydisperse	Polydisperse	Monodisperse	Monodisperse	Monodisperse
Particle Size	1.5-5μm	0.2-2μm	1-1.5μm	0.2-1.5μm	0.8-1μm
Color	Black	Yellowish Brown	Dark Brown	Dark Brown	Yellowish Brown
Magnetic response	15-30s	~60s	~30s	20s	120s
Settling Time (1ml)	>5min	>10min	>3min	>3min	>2h
Usage (0.2ml Sample)	20μl	20μl	20-30μl	20-30μl	20-30μl
DNA Recover Rate (only 4M GITC)	>80%	>80%	>80%	>80%	0
DNA Recover Rate (10% PEG8000/NaCl)	>85%	>85%	>85%	>85%	>90%
Recommended Use	gDNA/RNA Isolation from Blood, Tissue, Plant, Swab, Spots, Stool, Soil and etc. Viral DNA/RNA Isolation Agarose Gel DNA Purification	DNA/RNA Isolation from low nucleic acid content samples Plasmid Isolation DNA/RNA Clean Up	Circulating DNA Isolation Viral Nucleic acid Isolation gDNA Isolation FFPE DNA/RNA Isolation	Plasmid extraction gel DNA recovery genomicDNA/RNA extraction viral nucleic acid extraction Circulating DNA extraction	DNA/RNA Clean Up and concentration DNA/RNA Isolation from low nucleic acid content samples Research immuno assays

 

△ The MagPure magnetic-particle technology combines the speed and efficiency of silica-based DNA purification with the convenient handling of magnetic particles. DNA binds to the silica surface of the magnetic particles in the presence of a chaotropic salt. DNA bound to the particles is then efficiently washed, considerably improving the purity of DNA. High-quality DNA is eluted. The automated purification procedure completely removes enzymes, nucleotides, and other contaminants and inhibitors. Purified DNA is suitable for direct use in downstream applications, such as sequencing and microarray analysis.


 

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