Material: | Plate,Liquid,Powder |
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Transport Package: | Box |
Specification: | 25 Preps/Kit |
Trademark: | Magen Biotech |
Origin: | China |
Samples: |
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Customization: |
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Introduction
The HiPure Midi system provides a fast, simple, and cost-effective plasmid DNA midiprep method for routine molecular biology laboratory applications. HiPure Midiprep Kits use silica membrane technology to eliminate the cumbersome steps associated with loose resins or slurries. Plasmid DNA purified with Mini Kits is immediately ready for use. Phenol extraction and ethanol precipitation are not required, and high quality plasmid DNA is eluted in a small volume of Tris buffer or water.
Specifications
Features | Specifications |
Main Functions | Isolation up to 500µg endotoxin-free plasmid DNA from 25-50ml bacterial culture |
Applications | Cell transfection, animal injection, etc. |
Purification method | Midi spin column |
Purification technology | Silica technology |
Process method | Manual (centrifugation or vacuum) |
Sample type | Conventional plasmid, plasmid less than 30KB |
Sample amount | 25-50ml |
Yield | 10-500μg |
Elution volume | ≥0.4ml |
Time per run | ≤60 minutes |
Liquid carrying volume per column | 4ml |
Binding yield of column | 250μg |
Kit Contents
Contents | P123102 | P123102B |
Purification Times | 50 Preps | 50 Preps |
RNase A | 30 mg | 30 mg |
Buffer E1 | 150 ml | 150 ml |
Buffer E2 | 150 ml | 150 ml |
Buffer E3 | 150 ml | 150 ml |
Buffer E4 | 150 ml | 150 ml |
Buffer E5 | 150 ml | 150 ml |
Buffer PW2 | 50 ml | 50 ml |
Elution Buffer | 30 ml | 30 ml |
MaxPure EF Midi Column | 50 | 50 |
2 ml Collection Tubes | 50 | 50 |
Lysate Clear Midi Column | 50 | 50 |
Extend Tubes | 50 | 50 |
50ml Centrifuge Tubes | 50 | |
Support Tubes | 50 |
Storage and Stability
The kit components can be stored dry at room temperature (15-25°C) and are stable for at least 18 months under these conditions. If any precipitates form in the buffers, warm at 37ºC to dissolve. After addition of RNase A and optional LyseBlue reagent, Buffer P1 is stable for 6 months when stored at 2-8°C.
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