Hipure Sf Plant DNA Mini Kit

• Material: Plate,Liquid,Powder
• Transport Package: Box
• Specification: 50 Preps/Kit
• Trademark: Magen Biotech
• Origin: China
• Model NO.: D316402
• HS Code: 3822009090

Product Description

Product Description

Introduction
This product provides a fast and easy way to purify DNA from plant and fungal tissue. Up to 100 mg of tissue can be processed. Easy-to-use Plant procedures provide pure total DNA (genomic,mitochondrial, and chloroplast) for reliable PCR and southern blotting in less than 1 hour. Purification requires no phenol or chloroform extraction oralcohol precipitation and involves minimal handling.




Specifications

Features	Specifications
Main Functions	Isolation total DNA from100 mg simple plant
Applications	PCR, SSR, AFLP, RAPD and southern blot, etc.
Purification method	Mini spin column
Purification technology	Silica technology
Process method	Manual (centrifugation or vacuum)
Sample type	Economic plant samples
Sample amount	Fresh / frozen plant samples: ≤100m gDried plant / seed samples: ≤20mg
Elution volume	≥30μl
Time per run	30-50 minutes
Liquid carrying volume per column	800μl
Binding yield of column	100μg

Principle
Plantmaterial is first mechanically disrupted and then lysed by addition of lysis buffer and incubation. RNase A in the lysis buffer digests the RNA in the sample. After lysis, proteins and polysaccharides are salt-precipitated.Binding buffer and ethanol are added to the cleared lysate to promote binding of the DNA to the HiPure membrane.

The sample is then applied to a column and then centrifuged. DNA binds to the membrane, while contaminants such as proteins and polysaccharides are efficiently removed by 2 wash steps. Pure DNA is eluted in a small volume of low-salt buffer or water.




 

Advantages

• Safe - require nophenol or chloroform extraction
• Fast - several samples can be extracted in 30 minutes by silica technology
• High purity - high quality DNA, completely remove inhibitors
• High yield - silica technology can achieve the highest yield

Kit Contents

Contents	D316402	D316403
Purification Times	50 Preps	250 Preps
RNase A	10 mg	50 mg
Protease Dissolve Buffer	1.8 ml	5 ml
Buffer SPL	30 ml	150 ml
Buffer PS	10 ml	50 ml
Buffer GW1	22 ml	110 ml
Buffer GW2*	12 ml	2 x 50 ml
Buffer AE	15 ml	60 ml
HiPure gDNA Mini Columns	50	2 x 125
2 ml Collection Tubes	50	2 x 125

 

Storage and Stability
Nase A should be stored at 2-8°C upon arrival. However, short-term storage (up to 24 weeks) at room temperature (15-25°C) does not affect its performance. The remaining kit components can be stored dry at room temperature (15-25°C) and are stable for at least 18 months under these conditions.

Experiment Data



 

 

 

Packaging & Shipping

Certificate

 

Laboratory

Workshop

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